New method for extracting heparin sodium bilirubin vitamin E
Heparin sodium is extracted from the lungs of pigs, cattle and sheep: it is to homogenize the animal's lungs into a slurry - slurry heat preservation enzyme solution - enzymatic hydrolysis solution to collect the filtrate - ion exchange adsorption treatment of the filtrate - resin washing and elution - clarification filtration - drying Heparin sodium. The extraction method adopts enzymatic hydrolysis and resin purification. Compared with the traditional salt solution method, the product titer can be increased by 15%-20%, and the yield is increased by more than 10%; the raw materials are low-cost and easy to obtain pigs, cattle and sheep. The lungs can greatly reduce the cost and are suitable for scale production; and reduce environmental pollution compared with the salt solution.
A new process for extracting heparin sodium by using a long-chain quaternary ammonium salt precipitation method without resin, which is obtained by salting, cetylpyridinium bromide complexation, washing, dissociation, filtration, precipitation, dehydration, and drying to obtain heparin sodium salt. The invention can increase the extraction rate of heparin sodium production by 30% compared with the resin adsorption method, and obtain a heparin sodium titer of 90-110 units/mg per kilogram of pig intestinal mucosa, and the yield is 0.8 g. The color is whiter and the cost is lower.
Production process for extracting heparin sodium by activated bio-enzymatic method: it is characterized in that the activated enzyme enzyme, trypsin, is used as a catalyst for cracking in the cracking process, and two important process conditions must be maintained in the process. , that is, the pH value is 8.5-9.0 and the hot state, the maximum temperature should reach 85-95 ° C, thus ensuring the cracking speed, the recovery rate is over 95%, which is beneficial to make full use of resources and improve economic benefits.
Production of heparin sodium by enzymatic hydrolysis: The present invention discloses a method for producing heparin sodium by a bio-fermentation process. The method uses 2709 protease as a catalyst, D204 strong basic anion exchange resin as ion exchange resin, and uses 150-180 mesh multi-layer sieve to filter, which effectively overcomes the long production cycle, incomplete hydrolysis and low yield of the original production process. The purity of the obtained product is low, and the generated wastewater seriously pollutes the environment. The use of this process to produce 100 million international units of heparin sodium requires only 1800-2000 pig intestinal mucosa, and the product purity is 80%/mg or more.
2970006-Production of heparin sodium by pig, cattle and sheep lung precipitation method: the invention discloses a process for extracting heparin sodium by using cetyltrimethyltrimethylammonium bromide ammonium precipitation method by using pig, cow and sheep lung instead of resin method . It is mediated by benzene, ammonium and alkali to mediate, decompose and precipitate heparin-protein complex precipitated with cetyl ammonium bromide and then centrifuged to precipitate heparin. Thereby, the operation process for producing heparin sodium is reduced, and the extraction rate is increased.
A method for extracting bilirubin by using animal bile as a raw material: its main process is to mix animal bile, deionized water, dichloromethane and a small amount of antioxidant according to volume ratio, and add hydrochloric acid and acetic acid mixture under stirring condition. The pH is adjusted to an acidic range and then reacted at a normal temperature. After filtration, separation, concentration in a water bath at 60-80 ° C, crystallization, filtration and drying, the product is obtained. The invention has the advantages of short production process, small environmental pollution, less loss of solvent and bilirubin, no flocculation, low cost and high yield and purity of the product.
Enzymatic synthesis of bilirubin: the use of animal blood as a material to synthesize bilirubin by enzymatic method. Enzymatic degradation of synthetic bilirubin is carried out by centrifuging fresh animal blood, breaking cells into hemoglobin slurry, and adding a certain amount of recombinant heme oxygenase mixture to be hydrolyzed in a reaction column at 37 ° C for 5 hours. Adding sodium hydroxide to alkaline hydrolysis, removing protein by centrifugation, extracting the supernatant with chloroform, evaporating and concentrating, washing to produce bilirubin, shortening the production cycle of bilirubin synthesized by this method by two-thirds, and reducing the cost by 70%. The rate is up to one thousandth of the total blood volume, the hemoglobin conversion rate is more than 65%, the product purity is high, the bilirubin IXa content is as high as 92% or more, and the economic benefit is remarkable.
Method for rapidly extracting bilirubin: The invention discloses a method for extracting bilirubin, which comprises the following steps: adding sodium hydroxide solution to fresh bile, making the pH 10-11, heating to boiling; cooling to Add bisulfite and chloroform to the bile at room temperature, add hydrochloric acid to make the pH 5-6, and separate the solution with a separating funnel; separate the chloroform bilirubin solution and heat it in a water bath while recovering chloroform; The bilirubin after recovering chloroform is dissolved in alcohol; the bilirubin alcohol solution is evaporated and filtered to obtain bilirubin. The advantages of this method are simple process, high speed, high yield and low cost.
New technology for the production of bilirubin by blood decomposition: The present invention relates to the field of biochemical technology. The invention treats the blood of the livestock through a physical chemical method such as hypotonicity and strong alkali, so that it is hemolyzed, the red blood cells are destroyed, the hemoglobin is hydrolyzed, the hemoglobin is decomposed, and the biliverdin produced by the decomposition of the heme is reduced into the bilirubin. The process of extracting bilirubin from the solution of the reaction. The technology is characterized by red blood cell destruction. The three processes of hemoglobin hydrolysis and heme decomposition are completed in one chemical reaction.
Method for rapidly extracting bilirubin at high content: The present invention is an extraction method of bilirubin. That is, using sodium hydroxide solution to react with bilirubin conjugate in bile to form bilirubin sodium salt under boiling condition, adding chloroform and antioxidant under room temperature, adjusting pH with dilute hydrochloric acid, and the resulting bilirubin is soluble in chloroform. in. Distillation of chloroform, ethanol removal, filtration to obtain a bilirubin product with a content of about 90%, yield four to seven ten thousand, the extraction cycle is about 1.5 hours.
The high-effect closed method for the production of bilirubin technology is a major improvement over traditional manufacturing techniques. The encapsulation technique of the present invention enables the addition and chemical-physical reactions to be carried out in a closed state. Effectively prevent the volatilization of chloroform and other chemical agents, no environmental pollution, and ensure the safety of operators. The recovery rate of chloroform is over 92%, the repeated use rate of ethanol is over 80%, the yield of one-time process is more than 5__, the content is not less than 80%, and the yield of 200 ml of bile is completed in 40 minutes.
Direct and rapid extraction of bilirubin: bilirubin is an important biochemical drug. It is also an important raw material for artificially synthesizing bezoar. In the past, the method of extracting bilirubin was first alkalized into a bile calcium salt, and then acidified and reduced for extraction. The present invention is a method of direct extraction without complicated reactions. That is, an antioxidant, water and an organic solvent are added to the bile, and the appropriate pH is adjusted, heated and stirred, separated, distilled, and then precipitated with alcohol. The extraction cycle was shortened from four days to three to four hours. And the yield is increased by about 25%, and the purity can reach more than 90%.
Pig blood extraction heme technology: one liter of blood can extract 10-15 grams of hemoglobin, and can produce about 400 grams of protein at the same time, the processing process is simple, the cost is low.
Bilirubin extraction technology: The equipment for extracting bilirubin is simple, the technology is easy to learn, the investment is small, and the benefit is high.
The technology of extracting glycerin from waste distiller's grains: There are hundreds of thousands of wineries in China. Apart from feeding a small amount of poultry and agricultural fertilizers, the waste distiller's grains are not well utilized, which pollutes the environment and causes great waste. The technology of extracting glycerol (glycerol) from waste distiller's grains is tested by the State Administration of Commodity Inspection, and the indicators meet the prescribed standards (purity is 96.8%). Production of this product can be simple and simple, if you build a small three-factory, you only need to invest 2,000 yuan, 50 square meters of factory buildings, can handle 1000kg of distiller's grains (profit of 800 yuan). Because this product is an indispensable dry raw material for the medical, printing, leather, tobacco and other industries, and two-thirds of China's glycerin demand is dependent on imports, and prices are rising. Therefore, there is no need to worry about market sales.
Process for extracting vitamin E from vegetable oil deodorized distillate: including methyl esterification, water washing, depurination, removal of fatty acid methyl ester, cold precipitation de-solidification, distillation, especially after two methyl esterifications, water washing, Dehydration and transesterification are carried out. Transesterification is the lower alcohol required to transfer the dehydrated mixture into the reaction vessel, adding the catalyst and performing the transesterification reaction. The actual alcohol addition amount is 10-15 times of the theoretical requirement, and the reaction temperature is the reflux temperature of the alcohol 65-68 °C. The stirring reaction time is 1 to 2 hours. After the reaction is completed, an equal amount of concentrated sulfuric acid is added to neutralize the basic catalyst, and the reaction is terminated. The transesterification catalyst uses 50% or more of sodium methoxide in an amount of 5 ‰ to 2% of the neutral oil content. The extraction pressure is 10-18 Mp, and the extraction temperature is 32-55 ° C, and the fatty acid methyl ester is continuously extracted by supercritical carbon dioxide fluid.
A new process for extracting natural vitamin E and plant sterol from vegetable oil refining by-products: the process includes esterification, water washing, alcoholysis, cryogenic precipitation, distillation and the like. The invention converts a neutral oil into a fatty acid methyl ester (or ethyl ester) by an alcoholysis reaction, and replaces the molecular distillation device with a simple vacuum distillation device to form a completely new process, thereby realizing the extraction and purification of the natural vitamin E, and simultaneously Get high quality phytosterol products.
Process for separating vitamin E from refined vegetable oil residue: the process consists of crude extraction and refining, including crude esterification, water washing, vacuum distillation, secondary esterification, secondary water washing, secondary vacuum distillation, extraction and cold analysis. Purification involves adsorption with a silica gel adsorption column and treatment with an anion exchange resin. The present invention can obtain vitamin E at a concentration of 50 to 60% from vegetable oil residue, and the concentration of vitamin E can be widely used in the pharmaceutical, food and feed industries. The process of the invention is simple and easy to operate, and the economic benefits are obvious.
Process for extracting vitamin E from vegetable oil deodorized distillate: including methyl esterification, water washing, depurination, removal of fatty acid methyl ester, cold precipitation de-solidification, distillation, especially after two methyl esterifications, water washing, Dehydration and transesterification are carried out. Transesterification is the lower alcohol required to transfer the dehydrated mixture into the reaction vessel, adding the catalyst and performing the transesterification reaction. The actual alcohol addition amount is 10-15 times of the theoretical requirement, and the reaction temperature is the reflux temperature of the alcohol 65-68 °C. The stirring reaction time is 1 to 2 hours. After the reaction is completed, an equal amount of concentrated sulfuric acid is added to neutralize the basic catalyst, and the reaction is terminated. The transesterification catalyst uses 50% or more of sodium methoxide in an amount of 5 ‰ to 2% of the neutral oil content. The extraction pressure is 10-18 Mp, and the extraction temperature is 32-55 ° C, and the fatty acid methyl ester is continuously extracted by supercritical carbon dioxide fluid.
Tear Resistance Tester,Custom Elmendorf Tear Tester,Elmendorf Tear Strength Tester,Digital Tear Resistance Tester
Dongguan Hengke Automation Equipment Co., Ltd. , https://www.hengkeinstrument.com