2CY-12 / 2.5 gear pump the correct procedure steps
First, 2CY-12 / 2.5 gear pump before the start of preparation: 1. Test the motor steering is accurate (with the pump body marked on the direction), the test time is shorter to avoid the pump internal wear and tear on the wear parts due to dry lubrication without lubrication damage ; 2. Open the exhaust valve so that the liquid covered the entire pump, to be closed after the exhaust valve; 3. Check the various parts are normal; 4. High temperature type should be to enhance predecessor line preheating, heating rate of 50 ℃ / Ensure that all parts of the average heat. Second, start: 1. Close the pump outlet valve; 2. Fully open the pump inlet valve; 3. Start the motor to observe the pump is running properly; 4. Adjust the outlet valve opening to the required conditions, such as users at the pump outlet installed A flow meter or pressure gauge, the valve should be adjusted by adjusting the degree of opening of the pump performance parameters listed in the table rated point on the operation, such as the user at the pump outlet is not installed flow meter or pressure gauge should be adjusted by the outlet valve opening ; Measure the pump motor current, the motor running within the rated current, otherwise it will cause the pump to overload operation (ie, high current operation, to make the motor burnout) Adjust the outlet valve open large and small and pipeline conditions; 5. Check the seal leakage, the normal mechanical seal leakage should be less than 3 drops / min; 6. Check the motor, bearing temperature rise ≤ 80 ℃. Third, downtime: 1. Closed export pipeline valves; 2. Intercept power; 3. Closed the entrance pipeline valves.
2. Make the specimen relatively fixed;
3. It is convenient to use the capillary phenomenon to form a gradient when adding various reagents (staining agent, acid, salt solution);
4. Avoid contamination of the specimen.
The cover glass is covered on the material on the glass slide, which can avoid the contact between the liquid and the objective lens, so as not to contaminate the lens, and can make the top of the observed cells in the same plane, that is, the distance from the objective lens is the same, so that the observed Images are clearer.Why do you need a coverslip when using a microscope
1. Make the object to be observed form a thin film, which is easy to transmit light and easy to observe;2. Make the specimen relatively fixed;
3. It is convenient to use the capillary phenomenon to form a gradient when adding various reagents (staining agent, acid, salt solution);
4. Avoid contamination of the specimen.
The purpose of staining the specimen with iodine solution is to make the cells more obvious under the microscope, and it can also kill the cells and prevent the cells from migrating.
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